{"id":8519,"date":"2022-10-14T07:20:51","date_gmt":"2022-10-13T23:20:51","guid":{"rendered":"https:\/\/www.xiaoxianmi.cn\/?p=8519"},"modified":"2022-10-14T07:26:16","modified_gmt":"2022-10-13T23:26:16","slug":"%e8%a1%a8%e9%9d%a2%e6%b4%bb%e6%80%a7%e5%89%82%e5%bc%ba%e5%8c%96%e8%b6%85%e5%a3%b0%e6%b3%a2%e6%8f%90%e5%8f%96%e5%89%a9%e4%bd%99%e6%b1%a1%e6%b3%a5%e4%b8%ad%e9%ab%98%e5%88%86%e5%ad%90%e7%89%a9%e8%b4%a8","status":"publish","type":"post","link":"https:\/\/www.xiaoxianmi.cn\/en\/8519.html","title":{"rendered":"\u8868\u9762\u6d3b\u6027\u5242\u5f3a\u5316\u8d85\u58f0\u6ce2\u63d0\u53d6\u5269\u4f59\u6c61\u6ce5\u4e2d\u9ad8\u5206\u5b50\u7269\u8d28"},"content":{"rendered":"<p><strong>\u6587\u7ae0\u4eae\u70b9<\/strong><\/p>\n<ul>\n<li>\u9996\u6b21\u63d0\u51fa\u81ea\u5269\u4f59\u6c61\u6ce5\u4e2d\u540c\u6b65\u56de\u6536\u80de\u5185\u4e0e\u80de\u5916\u9ad8\u5206\u5b50\u7269\u8d28<\/li>\n<li>\u9ad8\u6548\u3001\u65e0\u6bd2\u3001\u53ef\u751f\u7269\u964d\u89e3\u7684CTAB\u4e3a\u5907\u9009\u8868\u9762\u6d3b\u6027\u5242<\/li>\n<li>CTAB\u663e\u8457\u5f3a\u5316\u8d85\u58f0\u6cd5\u63d0\u53d6\u9ad8\u5206\u5b50<\/li>\n<li>\u56de\u6536\u7684\u9ad8\u5206\u5b50\u4e2dAl\u3001Na\u3001Ca\u542b\u91cf\u663e\u8457\u964d\u4f4e<\/li>\n<li>\u56de\u6536\u7684\u9ad8\u5206\u5b50\u5bf9\u91cd\u91d1\u5c5e\u79bb\u5b50\u5438\u9644\u6027\u80fd\u53ef\u4e0e\u5546\u7528\u5438\u9644\u5242\u5ab2\u7f8e<\/li>\n<\/ul>\n<p><strong>\u6587\u7ae0\u7b80\u4ecb<\/strong><\/p>\n<p>\u6c61\u6c34\u8d44\u6e90\u5316\u662f\u672a\u6765\u6c61\u6c34\u5904\u7406\u7684\u53d1\u5c55\u65b9\u5411\uff0c\u4e5f\u662f\u4eba\u7c7b\u53ef\u6301\u7eed\u53d1\u5c55\u7684\u5fc5\u7136\u3002\u81ea\u6c61\u6c34\u6216\u6c61\u6ce5\u4e2d\u63d0\u53d6\u7684\u591a\u7cd6\u3001\u86cb\u767d\u8d28\u3001\u8150\u6b96\u8d28\u3001\u6838\u9178\u3001DNA\u3001\u78f7\u8102\u3001\u7cd6\u919b\u9178\u548c\u77ff\u7269\u8d28\u7b49\uff0c\u53ef\u4f5c\u4e3a\u5370\u67d3\u539f\u6599\u3001\u9996\u9970\u548c\u670d\u88c5\u539f\u6599\u3001\u963b\u71c3\u6750\u6599\u3001\u571f\u58e4\u8c03\u8282\u5242\u3001\u5438\u9644\u5242\u3001\u751f\u7269\u7d6e\u51dd\u5242\u3001\u9972\u6599\u6dfb\u52a0\u5242\uff0c\u5e94\u7528\u4e8e\u6c34\u5904\u7406\u3001\u519c\u4e1a\u3001\u56ed\u827a\u3001\u9020\u7eb8\u5de5\u4e1a\u3001\u533b\u7597\u3001\u5efa\u7b51\u5de5\u4e1a\u7b49\u9886\u57df\uff0c\u5177\u6709\u91cd\u5927\u7684\u56de\u6536\u4ef7\u503c\u3002<\/p>\n<p>\u5269\u4f59\u6c61\u6ce5\u4e2d\u5fae\u751f\u7269\u79cd\u7c7b\u7e41\u591a\uff0c\u56e0\u6b64\uff0c\u4ece\u4e2d\u63d0\u53d6\u7684\u7269\u8d28\u5fc5\u7136\u662f\u5404\u79cd\u9ad8\u5206\u5b50\u7269\u8d28\u7684\u6df7\u5408\u7269\uff0c\u65e0\u8bba\u662f\u80de\u5185\u8fd8\u662f\u80de\u5916\u9ad8\u5206\u5b50\u7269\u8d28\uff0c\u5b83\u4eec\u5747\u5177\u6709\u5178\u578b\u7684\u6d3b\u6027\u57fa\u56e2\uff0c\u5982\u7fa7\u57fa\u3001\u7f9f\u57fa\u3001\u78f7\u9178\u57fa\u7b49\u5b98\u80fd\u56e2\uff0c\u8fd9\u4e9b\u57fa\u56e2\u7684\u5b58\u5728\u53ef\u4f7f\u63d0\u53d6\u7684\u9ad8\u5206\u5b50\u7269\u8d28\u4f5c\u4e3a\u91cd\u91d1\u5c5e\u79bb\u5b50\u5438\u9644\u5242\u52a0\u4ee5\u5e94\u7528\u3002\u6545\uff0c\u56e2\u961f\u9996\u6b21\u63d0\u51fa\u4ece\u5269\u4f59\u6c61\u6ce5\u4e2d\u540c\u6b65\u56de\u6536\u80de\u5916\u805a\u5408\u7269\uff08Extracellular polymeric substances\uff0cEPSs\uff09\u548c\u80de\u5185\u805a\u5408\u7269\uff08Intracellular polymeric substances\uff0cIPSs\uff09\uff0c\u5373\u6df7\u5408\u7684\u9ad8\u5206\u5b50\u7269\u8d28\u3002<\/p>\n<p>\u8d85\u58f0\u6ce2\u4f5c\u4e3a\u4e00\u9879\u6e05\u6d01\u7684\u5904\u7406\u6280\u672f\uff0c\u4f5c\u7528\u8fc7\u7a0b\u4e2d\u4e0d\u9700\u8981\u6dfb\u52a0\u5316\u5b66\u836f\u5242\uff0c\u4e0d\u4f1a\u5e26\u6765\u4e8c\u6b21\u6c61\u67d3\u3002\u8d85\u58f0\u6ce2\u53ef\u4f7f\u7d6e\u4f53\u5728\u6c34\u76f8\u4e2d\u5206\u6563\u5f00\u6765\uff0c\u4e14\u7a7a\u5316\u6548\u5e94\u53ef\u5c06\u5927\u9897\u7c92\u7269\u8d28\u7834\u788e\u6210\u5c0f\u5c3a\u5bf8\u9897\u7c92\u7269\u3002\u8d85\u58f0\u6ce2\u6240\u4ea7\u751f\u7684\u526a\u5207\u529b\u548c\u7a7a\u7a74\u51b2\u51fb\u538b\u529b\uff0c\u7834\u788e\u6c61\u6ce5\u4e2d\u751f\u7269\u7d6e\u4f53\u7ed3\u6784\uff0c\u4f7f\u7ec6\u80de\u58c1\u7834\u88c2\uff0c\u4ece\u800c\u4fc3\u8fdb\u7ec6\u80de\u5185\u7269\u8d28\u7684\u91ca\u653e\uff1b\u540c\u65f6\uff0c\u8fd8\u53ef\u4fc3\u8fdb\u60ac\u6d6e\u7269\u6eb6\u89e3\uff0c\u4f7f\u4e0d\u6eb6\u6027\u6709\u673a\u9ad8\u5206\u5b50\u7269\u8d28\u8f6c\u5316\u4e3a\u53ef\u6eb6\u6027\u6709\u673a\u7269\uff1b\u6b64\u5916\uff0c\u8d85\u58f0\u6ce2\u8fd8\u80fd\u6539\u53d8\u6c61\u6ce5\u7684\u7c92\u5ea6\u3001\u8131\u6c34\u6027\u548c\u751f\u7269\u964d\u89e3\u6027\u7b49\u3002<\/p>\n<p>\u867d\u7136\u8d85\u58f0\u6ce2\u6cd5\u80fd\u591f\u6709\u6548\u5730\u7834\u788e\u7ec6\u80de\uff0c\u5177\u6709\u6548\u7387\u9ad8\u3001\u8bbe\u5907\u7b80\u5355\u3001\u64cd\u4f5c\u65b9\u4fbf\u7684\u4f18\u70b9\uff0c\u4f46\u662f\u5b83\u662f\u4e00\u79cd\u80fd\u6e90\u5bc6\u96c6\u578b\u7684\u5904\u7406\u65b9\u6cd5\uff0c\u6700\u5927\u7684\u7f3a\u70b9\u5c31\u662f\u8fd0\u884c\u80fd\u8017\u9ad8\uff0c\u4ece\u800c\u9650\u5236\u4e86\u5b9e\u9645\u5e94\u7528\u7684\u53ef\u80fd\u6027\u3002\u8868\u9762\u6d3b\u6027\u5242\u5177\u6709\u589e\u6eb6\u4f5c\u7528\uff0c\u53ef\u4ee5\u6d17\u8131\u6389\u7ec6\u80de\u819c\u4e0a\u7684\u86cb\u767d\u8d28\uff0c\u901a\u8fc7\u5f71\u54cd\u6e17\u900f\u538b\u4ece\u800c\u589e\u52a0\u7ec6\u80de\u819c\u7684\u901a\u900f\u6027\uff0c\u52a0\u5267\u5bf9\u6c61\u6ce5\u7d6e\u4f53\u7684\u7834\u574f\u3002\u6b64\u5916\uff0c\u8868\u9762\u6d3b\u6027\u5242\u4f5c\u7528\u53ef\u4fc3\u8fdb\u4ece\u5269\u4f59\u6c61\u6ce5\u4e2d\u63d0\u53d6EPS\uff0c\u6539\u53d8\u7ec6\u80de\u7ed3\u6784\u5e76\u5f71\u54cd\u6c61\u6ce5\u7279\u6027\u3002<\/p>\n<p>\u8868\u9762\u6d3b\u6027\u5242\u5f3a\u5316\u8d85\u58f0\u6ce2\u6cd5\u53ef\u7528\u4e8e\u63d0\u53d6\u5929\u7136\u9ad8\u5206\u5b50\u6750\u6599\uff0c\u4e5f\u53ef\u4f5c\u4e3a\u4e00\u79cd\u9884\u5904\u7406\u65b9\u6cd5\uff0c\u4ee5\u964d\u4f4e\u80fd\u8017\u3002\u672c\u7814\u7a76\u4ece\u56de\u6536\u5177\u6709\u5178\u578b\u5b98\u80fd\u56e2\u7684\u6df7\u5408PSs\u7684\u89d2\u5ea6\u51fa\u53d1\uff0c\u63d0\u51fa\u8868\u9762\u6d3b\u6027\u5242\u5f3a\u5316\u8d85\u58f0\u6ce2\u6cd5\u540c\u6b65\u63d0\u53d6\u5269\u4f59\u6c61\u6ce5\u4e2d\u7684EPSs\u548cIPSs\uff0c\u4f18\u5316\u5f97\u51fa\u6700\u4f73\u5de5\u827a\u6761\u4ef6\uff0c\u5e76\u8ba8\u8bbaPSs\u7684\u6750\u6599\u6027\u80fd\u3001\u91cd\u91d1\u5c5e\u79bb\u5b50\u7684\u5438\u9644\u6027\u80fd\u4ee5\u53ca\u63d0\u53d6\u524d\u540e\u6c61\u6ce5\u7684\u8fc7\u6ee4\u8131\u6c34\u6027\u80fd\u3002\u672c\u7814\u7a76\u53ef\u4e3a\u672a\u6765\u6c61\u6c34\u5904\u7406\u6280\u672f\u4ee5\u53ca\u6c61\u6c34\u8d44\u6e90\u5316\u63d0\u4f9b\u601d\u8def\uff0c\u4ece\u800c\u6700\u5927\u9650\u5ea6\u5730\u63d0\u9ad8\u5269\u4f59\u6c61\u6ce5\u7684\u5229\u7528\u7387\uff0c\u6210\u679c\u4e8e2021\u5e749\u6708\u53d1\u8868\u5728\u300aChemosphere\u300b\u6742\u5fd7\uff0c\u76f8\u5173\u6280\u672f\u5df2\u83b7\u56fd\u5bb6\u53d1\u660e\u4e13\u5229\u6388\u6743\uff08\u4e13\u5229\u53f7\uff1aZL201910739281.5\uff09\u3002<\/p>\n<p><strong>\u4e3b\u8981\u7ed3\u679c<\/strong><\/p>\n<p>\u8868\u9762\u6d3b\u6027\u5242\u5f3a\u5316\u8d85\u58f0\u6cd5\u662f\u4ece\u5269\u4f59\u6c61\u6ce5\u4e2d\u56de\u6536PSs\u7684\u4e00\u79cd\u5177\u6709\u4f18\u52bf\u7684\u63d0\u53d6\u65b9\u6cd5\u3002\u4f18\u5316\u80fd\u8017\u4e0e\u63d0\u53d6\u6548\u7387\u540e\uff0c\u5f53\u8d85\u58f0\u529f\u7387\u4e3a100 W\u3001\u6709\u6548\u8d85\u58f0\u65f6\u95f4\u4e3a5 min\uff0c\u79bb\u5fc3\u901f\u5ea6\u4e3a5145 r\u00b7min-1\u3001\u5341\u516d\u70f7\u57fa\u4e09\u7532\u57fa\u6eb4\u5316\u94f5\uff08CTAB\uff09\u6d53\u5ea6\u4e3a0.1 g\u00b7L-1\u65f6\uff0cPSs\u7684\u63d0\u53d6\u6548\u7387\u6700\u9ad8\uff0c\u8f83\u8d85\u58f0\u6ce2\u6cd5\u9ad876.5%\u3002CTAB\u76f8\u8f83\u4e8eSDS\u65e0\u6bd2\u6027\u4e14\u53ef\u751f\u7269\u964d\u89e3\uff0c\u53ef\u51cf\u8f7b\u63d0\u53d6PSs\u540e\u4ea7\u751f\u6b8b\u7559\u7269\u7684\u5904\u7406\u8d1f\u62c5\u3002\u7531\u4e8e\u8d85\u58f0\u6ce2\u4f7f\u7ec6\u80de\u7834\u788e\uff0c\u8868\u9762\u6d3b\u6027\u5242\u5f3a\u5316\u8d85\u58f0\u6ce2\u6cd5\u5bf9\u542b\u6709IPSs\u548cEPSs\u7684PSs\uff08tp = 5 min\uff0cCCTAB= 0.1 g\u00b7L-1\uff09\u7684\u63d0\u53d6\u6548\u7387\u8fdc\u9ad8\u4e8e\u9633\u79bb\u5b50\u4ea4\u6362\u6811\u8102\uff08CER\uff09\u6cd5\u3002<\/p>\n<p>\u8868\u9762\u6d3b\u6027\u5242\u5f3a\u5316\u8d85\u58f0\u4f5c\u7528\u4e0b\u5269\u4f59\u6c61\u6ce5\u4e2dPSs\u63d0\u53d6\u91cf\u7684\u589e\u52a0\u662f\u7531\u4e8e\u7d6e\u4f53\u89e3\u4f53\u548c\u7ec6\u80de\u6eb6\u89e3\u5bfc\u81f4\u6709\u673a\u7269\u91ca\u653e\u3002PSs\u7684\u91ca\u653e\u673a\u5236\u53ef\u80fd\u5305\u62ec\u4ee5\u4e0b\u56db\u4e2a\u673a\u7406\u4e2d\u7684\u4e00\u4e2a\u6216\u591a\u4e2a\uff1a<\/p>\n<ul>\n<li>\u8868\u9762\u6d3b\u6027\u5242\u964d\u4f4e\u6eb6\u6db2\u7684\u8868\u9762\u5f20\u529b\uff0c\u52a0\u5267\u8d85\u58f0\u6ce2\u4f5c\u7528\u65f6\u7684\u7a7a\u5316\u6548\u5e94\uff0c\u4fc3\u8fdb\u80de\u5185\u5916\u9ad8\u5206\u5b50\u7684\u8131\u9644\uff0c\u4ece\u800c\u964d\u4f4e\u8d85\u58f0\u6ce2\u8fc7\u7a0b\u7684\u80fd\u91cf\u6d88\u8017\uff1b<\/li>\n<li>\u8868\u9762\u6d3b\u6027\u5242\u7834\u574f\u4e86EPSs\u4e0e\u5fae\u751f\u7269\u7ec6\u80de\u4f53\u95f4\u7684\u975e\u5171\u4ef7\u952e\uff0c\u4ece\u800c\u5bfc\u81f4EPSs\u4ece\u7ec6\u80de\u819c\u8868\u9762\u8131\u9644\uff1b<\/li>\n<li>\u7531\u4e8e\u76f8\u4f3c\u76f8\u6eb6\u539f\u7406\uff0c\u8868\u9762\u6d3b\u6027\u5242\u589e\u52a0\u591a\u7cd6\u3001\u86cb\u767d\u8d28\u7b49\u9ad8\u5206\u5b50\u7684\u6eb6\u89e3\u5ea6\uff1b<\/li>\n<li>\u8868\u9762\u6d3b\u6027\u5242\u4e2d\u6781\u6027\u5b98\u80fd\u56e2\u7834\u574f\u7ec6\u80de\u819c\u6216\u7ec6\u80de\u58c1\u7ed3\u6784\u5982\u78f7\u8102\u53cc\u5206\u5b50\u5c42\uff0c\u5bfc\u81f4\u7ec6\u80de\u88c2\u89e3\u3001IPSs\u91ca\u653e\u3002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>PSs\u4e2d\u86cb\u767d\u8d28\u7684\u767e\u5206\u542b\u91cf\u9ad8\u4e8e\u591a\u7cd6\uff0c\u591a\u7cd6\u3001\u86cb\u767d\u8d28\u548cDNA\u7684\u767e\u5206\u542b\u91cf\u7ea6\u5360PSs\u603b\u91cf\u768450%\u3002\u8868\u9762\u6d3b\u6027\u5242\u6d53\u5ea6\u7684\u53d8\u5316\u5bf9PSs\u4e2d\u5404\u9ad8\u5206\u5b50\u7684\u767e\u5206\u542b\u91cf\u5f71\u54cd\u8f83\u5c0f\uff0c\u8fd9\u53ef\u80fd\u662f\u56e0\u4e3aEPS\u548cIPS\u4e2d\u5404\u7ec4\u5206\u7684\u6784\u6210\u662f\u76f8\u4f3c\u7684\u3002DNA\u7684\u767e\u5206\u6bd4\u4fdd\u6301\u6052\u5b9a\uff0c\u8868\u660e\u76f8\u5e94\u6761\u4ef6\u4e0bCTAB\u6d53\u5ea6\u7684\u589e\u52a0\u5e76\u6ca1\u6709\u4fc3\u8fdb\u5269\u4f59\u6c61\u6ce5\u4e2d\u7ec6\u80de\u7684\u88c2\u89e3\u3002\u8868\u9762\u6d3b\u6027\u5242\u5f3a\u5316\u8d85\u58f0\u63d0\u53d6\u7684\u9ad8\u5206\u5b50\uff08\u8bb0\u4e3aPSus\uff09\u7684\u7c92\u5f84\u5c0f\u4e8e\u672a\u6dfb\u52a0\u8868\u9762\u6d3b\u6027\u5242\u63d0\u53d6\u7684\u9ad8\u5206\u5b50\uff08\u8bb0\u4e3aPSu\uff09\uff0c\u8868\u660e\u5269\u4f59\u6c61\u6ce5\u4e2d\u7c92\u5f84\u8f83\u5c0f\u7684EPS\u53ef\u4ee5\u7ecf\u8868\u9762\u6d3b\u6027\u5242\u5f3a\u5316\u8d85\u58f0\u6ce2\u63d0\u53d6\uff1b\u8fd9\u53ef\u80fd\u662f\u7531\u4e8e\u8868\u9762\u6d3b\u6027\u5242\u7684\u76f8\u4f3c\u76f8\u6eb6\u6027\u5bfc\u81f4\u5c0f\u7c92\u5f84\u7684IPSs\u91ca\u653e\uff0c\u4ee5\u53ca\u5c0f\u7c92\u5f84\u7684PSs\u6eb6\u89e3\u5ea6\u589e\u52a0\u3002PSus\u548cPSu\u5747\u5b58\u5728\u591a\u79cd\u7279\u5f81\u5b98\u80fd\u56e2\uff0c\u5982O\u2013H\u3001\u2013NH2\u3001C=O\u3001C\u2013N\u3001N\u2013H\u3001COO\u2013\u3001C\u2013O\u2013C\u3001C\u2013OH\u548cP\u2013O\uff0c\u6362\u8a00\u4e4b\uff0c\u4e24\u79cdPSs\u4e2d\u90fd\u542b\u6709\u86cb\u767d\u8d28\u3001\u591a\u7cd6\u3001\u8102\u8d28\u3001DNA\u548c\u82b3\u9999\u5316\u5408\u7269\u7b49\u9ad8\u5206\u5b50\uff0c\u6545\uff0c\u8868\u9762\u6d3b\u6027\u5242\u7684\u6dfb\u52a0\u5bf9\u8d85\u58f0\u6cd5\u4ece\u5269\u4f59\u6c61\u6ce5\u4e2d\u63d0\u53d6PSs\u7684\u7279\u6027\u5f71\u54cd\u8f83\u5c0f\u3002<\/p>\n<p>PSus\u548cPSu\u4e2dC\u3001O\u3001N\u548cP\u7684\u542b\u91cf\u5dee\u5f02\u8f83\u5c0f\uff0cPSus\u4e2d\u91d1\u5c5e\u542b\u91cf\u663e\u8457\u964d\u4f4e\uff08Al\uff1a0.18% \u21920\uff1bNa\uff1a0.15% \u21920\uff1bCa\uff1a0.24% \u21920.11%\uff09\uff0c\u53ef\u80fd\u662f\u7531\u4e8eCTAB\u4e0e\u5269\u4f59\u6c61\u6ce5\u4e2d\u7684\u91d1\u5c5e\u79bb\u5b50\u53d1\u751f\u4f5c\u7528\uff0cCTAB\u4f5c\u4e3a\u6c61\u6ce5\u7ec6\u80de\u8868\u9762\u5316\u5b66\u4fee\u9970\u5242\uff0c\u66ff\u4ee3\u7ec6\u80de\u8868\u9762\u758f\u6c34\u9634\u79bb\u5b50\u57fa\u56e2\uff0c\u7ed3\u5408\u7ec6\u80de\u8868\u9762\u7684\u9633\u79bb\u5b50\uff08Al3+\u3001Ca2+\u3001Na+\uff09\u3002\u503c\u5f97\u8bf4\u660e\u7684\u662f\uff0cEPS\u4e2d\u542b\u6709\u7684\u65e0\u673a\u7269\u5982\u91d1\u5c5e\u76d0\u7c7b\u5e38\u5e38\u88ab\u7814\u7a76\u8005\u6240\u5ffd\u89c6\uff0c\u65e0\u673a\u7269\u7684\u5b58\u5728\u5c06\u5f71\u54cdPSs\u7684\u7406\u5316\u6027\u8d28\uff0c\u6545PSus\u53ef\u80fd\u6bd4PSu\u5177\u6709\u66f4\u4f18\u8d8a\u7684\u6027\u80fd\u3002<\/p>\n<p>\u5206\u6790PSs\u7684\u7279\u5f81\u5b98\u80fd\u56e2\uff0cC\u2013(C, 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